7b postlab assignment

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Exercise 7B Postlab

Instructions:

  • Below is a list of the resources that you need to complete the Postlab Assignment for Exercise 7B.
  • The correctness and effort of your work determines your grade on this set of activities. 
  • This assignment should be completed individually.  Any assignments that have been duplicated or plagiarized (in part or in total) will not be accepted.  Because many of you may not have experience with these types of activities, a grading rubric exists for you to use.

Powerpoint Slides: In this part of the Postlab assignment, you continue to build your final presentation for the semester-long Wolbachia project.

  • Your final presentation for this course will include these slides that cover this information.
  • Refer to the Powerpoint “Style Guide” to earn a better grade!

Slide 1: Create a slide that contains an appropriate, descriptive title and a table that summarizes the expected results from your PCR activity.

  • Include an appropriate, descriptive title.
  • Refer to Table 6-4 of Exercise 6 in your lab manual as a guide for what to include (page 207).
  • DO NOT just copy Table 6-4. 
  • DO NOT include all of the information from the table. Evaluate which information in Table 6-4 is appropriate for this part of the assignment.  Any unnecessary information that is included will result in a deduction.
  • Use specimen IDs (do not use generic terms such as sample/unknown #1, #2, etc).
  • Don’t include “?” as expected results for Wolbachia for your insect specimen. You made a prediction based on the literature.  What do you expect based on your prediction?

Slide 2: Create a slide that contains a labelled figure of your gel that you began creating in Part five of Exercise 7B in lab (directions on page 260). To do this, take your gel picture and edit it in Powerpoint to include the following information on the slide for full credit:

  • an appropriate, descriptive title (Hint: Again, “Gel Electrophoresis” is NOT informative!)
  • an enlarged, cropped view of the original gel image, which should fill most of the slide
  • labels for gel lanes, ladder band sizes (including units), insect and Wolbachia gene names and true sizes (including units)
  • type of gel (2% of agarose) and voltage (100V for 45 minutes) used for electrophoresis.

Slide 3: Create a slide that includes the prediction, the evaluation of the prediction, and the relevance of the data to what is already known.

  • Include an appropriate, descriptive title.
  • Answer the question, “Did your data support your prediction or not?”
  • Compare your results to the information that you found about Wolbachia infection in your insect Order in the peer-reviewed literature and in the local collection data (from Exercise 3).

Slide 4: Create a slide that includes a detailed reflection that describes one benefit of your individual experience in this lab course.

  • Include an appropriate, descriptive title.
  • This is your opportunity to provide a thoughtful, unique reflection on your experience working through the Wolbachia project in this course.

Assignment Submission Instructions

Save and name your file

  1. Report your work as information in 4 MS Powerpoint slides. Details on what each of these slides should include can be found above.
  2. Check yourself using the associated rubric (Exercise 7B Postlab Assignment” Folio Rubric).  
  3. You may NOT submit Google Slides or any other software equivalent. If you prepare your slides in Google Slides, be sure to download as a MS Powerpoint file before uploading to the postlab dropbox.
  4. Save your assignment in the appropriate file format as “Exercise7BPostlab” followed by your three initials.  (For example, “Exercise7BPostlabGJD.pptx”)
  5. Upload and Submit your MS Powerpoint file to this dropbox (Exercise 7B Postlab Assignment Folio Dropbox Folder).

3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …

https://georgiasouthern.desire2learn.com/d2l/common/dialogs/nonModal/blank.d2l?d2l_body_type=1&d2l_nonModalDialog_cb=previewRubric_01245… 1/6

Exercise 7B Postlab Assignment Rubric
Course: Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2)

PCR Acceptable Developing Developing Developing Absent
Criterion
Score

Control and

Sample ID

Names

/ 3

Expected/Pr

edicted

Results

/ 10

Relevance

of

information

/ 5

3 points

All control and

sample ID

names are

included.

2 points

One sample or

control or

sample ID

name is

missing OR

sample names

are are not

properly listed

(e.g.

MEH12F18-

1).

1 point

More than one

control or

sample ID

name is

missing.

0 points 0 points

No control

names or

sample IDs are

included.

10 points

The

expected/pred

icted results

for all PCR

samples are

listed

correctly.

8 points

The

expected/pred

icted results

for ONE

control or

unknown PCR

sample is not

listed

corrected.

6 points

The

expected/pred

icted results

for TWO

control or

unknown PCR

samples are

not listed

corrected.

4 points

The

expected/pred

icted results

for MORE

THAN TWO

control or

unknown PCR

samples are

not listed

corrected.

0 points

No

expected/pred

icted results

are presented

correctly, or

the PCR slide

was not

included in the

student’s

submission.

5 points

All tabular

data are

relevant. No

unnecessary

information is

presented.

4 points

The table

includes ONE

additional

irrelevant item

(e.g. amounts

of water,

primers,

template, etc)

3 points

The table

includes TWO

additional

irrelevant item

(e.g. amounts

of water,

primers,

template, etc)

1 point

The table

includes

THREE or

more

additional

irrelevant item

(e.g. amounts

of water,

primers,

template, etc)

0 points

There was no

PCR slide

included in the

student’s

submission.

3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …

https://georgiasouthern.desire2learn.com/d2l/common/dialogs/nonModal/blank.d2l?d2l_body_type=1&d2l_nonModalDialog_cb=previewRubric_01245… 2/6

Gel

Electroph

oresis

Slide

Acceptable Developing Developing Developing Developing Absent
Criterion
Score

Lane

Labeling

/ 10

Ladder

Band

Labeling

/ 5

PCR

Product

Bands

/ 16

10 points

All lanes are

labeled

appropriatel

y.

8 points

One lane is

not labeled

appropriatel

y.

6 points

Two lanes

are not

labeled

appropriatel

y.

4 points

Three lanes

are not

labeled

appropriatel

y.

2 points

Four or more

lanes are not

labeled

appropriatel

y.

0 points

No lanes are

labeled.

5 points

The bands in

the DNA

ladder are

labeled

appropriatel

y.

4 points

The bands in

the DNA

ladder are

labeled but

at least ONE

of the labels

is not

appropriate

OR cannot

be easily

read (too

small or

crowded)

OR no units

are included.

2 points

The bands in

the DNA

ladder are

labeled but

at TWO-

THREE of

the labels is

not

appropriate

OR cannot

be easily

read (too

small or

crowded)

OR no units

are included.

1 point

The bands in

the DNA

ladder are

labeled but

at FOUR of

the labels

are not

appropriate

OR cannot

be easily

read (too

small or

crowded)

OR no units

are included.

0 points 0 points

No labeling

of the DNA

ladder is

presented.

16 points

Insect CO1

and

Wolbachia
16S rRNA

bands are

labeled

appropriatel

y.

12 points

At least

ONE-TWO

of the

following is

missing OR

labeled

incorrectly:

Insect CO1

label, 708bp,

Wolbachia
16S rRNA

label, 438bp.

8 points

THREE-

FOUR of the

following is

missing OR

labeled

incorrectly:

Insect CO1

label, 708bp,

Wolbachia

16S rRNA

label, 438bp.

4 points

MORE

THAN FOUR

of the

following is

missing OR

labeled

incorrectly:

Insect CO1

label, 708bp,

Wolbachia

16S rRNA

label, 438bp.

0 points 0 points

No labels on

the Insect

CO1 or

Wolbachia

16S rRNA

PCR

products are

presented.

3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …

https://georgiasouthern.desire2learn.com/d2l/common/dialogs/nonModal/blank.d2l?d2l_body_type=1&d2l_nonModalDialog_cb=previewRubric_01245… 3/6

Gel

Electroph

oresis

Slide

Acceptable Developing Developing Developing Developing Absent
Criterion
Score

Agarose

and

Voltage

/ 44 points

Gel

electrophore

sis

conditions

are

accurately

presented.

2 points

Either the

percentage

of agarose in

the gel OR

the voltage

is not

presented

accurately or

is missing.

0 points 0 points 0 points 0 points

Neither the

percentage

of agarose in

the gel nor

the voltage

is presented

OR both are

inaccurately

presented.

Conclusions/R

eflections
Acceptable Developing Developing Absent Criterion Score

Evaluation of

Prediction

/ 66 points

Presentation

clearly and

correctly

describes how

the results

support or do

not support the

prediction.

4 points

Student data are

not clearly linked

to the prediction,

and/or student

did not clearly

describe whether

the prediction

was supported or

not.

2 points

Student data are

not linked to the

prediction,

and/or student

did not clearly

describe whether

the prediction

was supported or

not.

0 points

Student did not

evaluate the

prediction after

completing the

experiment.

3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …

https://georgiasouthern.desire2learn.com/d2l/common/dialogs/nonModal/blank.d2l?d2l_body_type=1&d2l_nonModalDialog_cb=previewRubric_01245… 4/6

Conclusions/R

eflections
Acceptable Developing Developing Absent Criterion Score

Relevance of

Conclusions

/ 6

Reflection / 3

6 points

Relevance of

conclusions is

discussed as it

relates to

information

obtained from

peer-reviewed

scientific journal

articles and the

local collection

data.

4 points

An attempt is

made to discuss

the relevance of

the conclusions

as they relate to

data obtained

from peer-

reviewed

scientific journal

articles OR the

local collection

data.

2 points

An attempt is

made to discuss

the relevance of

the conclusions

as they relate to

data obtained

from peer-

reviewed

scientific journal

articles OR the

local collection

data but needs

significant

improvement.

0 points

Relevance of the

conclusions was

not discussed.

3 points

A thoughtful,

unique reflection

is presented in

detail.

2 points

A reflection is

presented but is

somewhat

detailed, unique,

or thoughtful.

1 point

A reflection is

presented but is

not detailed,

unique, or

thoughtful.

0 points

No reflection is

presented.

Presentation

Appearance
Acceptable Developing Absent Criterion Score

Slide Titles / 2
2 points

All slides have

appropriate,

descriptive titles.

1 point

One slide title is

missing OR One

slide title is

inappropriate.

0 points

Both slide titles are

missing OR Both

slide titles are

inappropriate OR

One slide title is

missing and the

other title is

inappropriate OR No

slide titles are

present.

3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …

https://georgiasouthern.desire2learn.com/d2l/common/dialogs/nonModal/blank.d2l?d2l_body_type=1&d2l_nonModalDialog_cb=previewRubric_01245… 5/6

Presentation

Appearance
Acceptable Developing Absent Criterion Score

Information Flow / 2

Amount of Text / 2

Font Size and

Style

/ 2

Spelling and

Grammar

/ 2

Image

Appearance

(Expected

Results)

/ 2

2 points

Information in the

slides is organized

and clearly relays

the expected or

actual results.

1 point

Information in the

slides is not well

organized or there is

additional

distracting

information included

(e.g., unnecessary

arrows, lines, or

animations)

0 points

Information in the

slides was poorly

organized and

unclear.

2 points

Appropriate amount

of text was included

on the slides.

1 point

Too much or too

little text was

included on ONE

slide.

0 points

Too much or too

little text was

included on BOTH

slides.

2 points

Font size is

appropriate. Font

style is consistent.

1 point

Slides show minor

issues with Fonts:

Font size is too

large, too small, or

inconsistent.

0 points

Font size is too

large, too small, or

inconsistent in

multiple areas of

slides.

2 points

No spelling or

grammar errors

exist.

1 point

Some spelling or

grammar problems

exist.

0 points

Many problems with

spelling and/or

grammar exist.

2 points

Expected results are

presented in tabular

format AND Table is

created using

PowerPoint

software.

1 point

Results are

presented but are

not in tabular format

OR Screenshot of

table from the lab

manual is inserted.

0 points

No expected results

are presented.

3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …

https://georgiasouthern.desire2learn.com/d2l/common/dialogs/nonModal/blank.d2l?d2l_body_type=1&d2l_nonModalDialog_cb=previewRubric_01245… 6/6

Total / 84

Overall Score

Presentation

Appearance
Acceptable Developing Absent Criterion Score

Image

Appearance (Gel

Results)

/ 2

Scientific Names / 2

2 points

Gel Image is a good

size, appropriately

cropped, focused

and not distorted.

1 point

The gel image had a

problem with ONE

of the following: not

the appropriate size,

not cropped

appropriately, not

clear (was

distorted).

0 points

The gel image had a

problem with MORE

THAN ONE of the

following: not the

appropriate size, not

cropped

appropriately, not

clear (was distorted)

OR Gel image was

not included.

2 points

Scientific names are

written correctly.

1 point

Scientific names are

written incorrectly.

0 points

No scientific names

are included.

A
75.6 points

minimum

B
67.2 points

minimum

C
58.8 points

minimum

D
50.4 points

minimum

F
0 points

minimum

Exercise 7B Postlab

Instructions:

· Below is a list of the resources that you need to complete the Postlab Assignment for Exercise 7B.

· The correctness and effort of your work determines your grade on this set of activities. 

· This assignment should be completed individually.  Any assignments that have been duplicated or plagiarized (in part or in total) will not be accepted.  Because many of you may not have experience with these types of activities, a grading rubric exists for you to use.

Powerpoint Slides: In this part of the Postlab assignment, you continue to build your final presentation for the semester-long Wolbachia project.

· Your final presentation for this course will include these slides that cover this information.

· Refer to the Powerpoint “Style Guide” to earn a better grade!

Slide 1: Create a slide that contains an appropriate, descriptive title and a table that summarizes the expected results from your PCR activity.

· Include an appropriate, descriptive title.

· Refer to Table 6-4 of Exercise 6 in your lab manual as a guide for what to include (page 207).

· DO NOT just copy Table 6-4. 

· DO NOT include all of the information from the table. Evaluate which information in Table 6-4 is appropriate for this part of the assignment.  Any unnecessary information that is included will result in a deduction.

· Use specimen IDs (do not use generic terms such as sample/unknown #1, #2, etc).

· Don’t include “?” as expected results for Wolbachia for your insect specimen. You made a prediction based on the literature.  What do you expect based on your prediction?

Slide 2: Create a slide that contains a labelled figure of your gel that you began creating in Part five of Exercise 7B in lab (directions on page 260). To do this, take your gel picture and edit it in Powerpoint to include the following information on the slide for full credit:

· an appropriate, descriptive title (Hint: Again, “Gel Electrophoresis” is NOT informative!)

· an enlarged, cropped view of the original gel image, which should fill most of the slide

· labels for gel lanes, ladder band sizes (including units), insect and Wolbachia gene names and true sizes (including units)

· type of gel (2% of agarose) and voltage (100V for 45 minutes) used for electrophoresis.

Slide 3: Create a slide that includes the prediction, the evaluation of the prediction, and the relevance of the data to what is already known.

· Include an appropriate, descriptive title.

· Answer the question, “Did your data support your prediction or not?”

· Compare your results to the information that you found about Wolbachia infection in your insect Order in the peer-reviewed literature and in the local collection data (from Exercise 3).

Slide 4: Create a slide that includes a detailed reflection that describes one benefit of your individual experience in this lab course.

· Include an appropriate, descriptive title.

· This is your opportunity to provide a thoughtful, unique reflection on your experience working through the Wolbachia project in this course.

Assignment Submission Instructions

Save and name your file

1. Report your work as information in 4 MS Powerpoint slides. Details on what each of these slides should include can be found above.

2. Check yourself using the associated rubric (
Exercise 7B Postlab Assignment” Folio Rubric
).  

3. You may NOT submit Google Slides or any other software equivalent. If you prepare your slides in Google Slides, be sure to download as a MS Powerpoint file before uploading to the postlab dropbox.

4. Save your assignment in the appropriate file format as “Exercise7BPostlab” followed by your three initials.  (For example, “Exercise7BPostlabGJD.pptx”)

5. Upload and Submit your MS Powerpoint file to this dropbox (
Exercise 7B Postlab Assignment Folio Dropbox Folder
).

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