Bio lab report

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explain the experiment and how it was performed so that i understand 

Enzymes: Form and Function

Catalytic proteins

Lower activation energy of reaction

Combine or break apart substrates

Not used up in reaction

End in –ase

Lactase metabolizes lactose

DNA Polymerase – polymerize nucleotides

Substrate to product


Energy of activation with enzyme

Energy needed to run reaction without an enzyme

Temp & pH

Changes in environmental conditions will alter enzyme structure, and therefore alter its function

Enzyme activity measured by

Initial reaction velocity (IRV)

Amount of product per time

this is what you’re doing in today’s experiment

Or could use amount of product per degree (for a temperature graph)

what is shown in the graph above

IRV = (slope of the graph)

Reaction you will run:

the reaction goes from clear to brown

peroxidase is not used up in reaction

guaiacol gets oxidized; hydrogen peroxide gets reduced

This is a redox reaction; Think about how rust turns a different color when iron is oxidized

Baseline: Filtered water – are the enzyme concentrations too weak/strong?

Question: how does pH impact the function of peroxidase?

Hypothesis: _______________________________________

Experiment: try at pH of 4, 7, and 10

guaiacol (clear) + H2O2 tetraguaiacol (brown) + H20




Make sure to only take out 300 microliters of Guaiacol

100 microliters to .1 milliliter

Get Guaiacol from me

Prepare your blank to tare the colorimeter – fill cuvette 2/3 full and make sure the clear side of cuvette is perpendicular to the colorimeter’s light

Don’t touch the clear sides (fingerprints are a problem).


Don’t turn upside down. 1st resistance to extract chemical; 2nd resistance to eject chemical. Pay attention to units on pipette. Don’t reuse tips.

Before adding enzyme solution to test, have all of your supplies ready:

your colorimeter tared and ready to go

Guaiacol, pH buffer, and H202 in a test tube


Once enzyme is added, quickly pour into cuvette and place in colorimeter, recording every 15 sec.

Input data into excel sheet for all of class to record

Calculate IRV using greatest difference between two adjacent points

You will make a line graph of our data using Excel (there will be 4 lines on graph)


We will cover components of the lab report following your lab practical next week

You will have access to sample lab reports, rubrics, instructions

Topic: Effects of pH on Peroxidase

upcoming due dates:

Materials and Methods – due March 15th 11:59pm on Canvas

Literature Review – due April 1st 11:59pm on Canvas

Introduction – due April 8th 11:59pm on Canvas

entire lab report – due April 22nd 11:59pm on Canvas

Lab Report – due March 25th
11:59pm on Canvas using Turnitin


covers labs: Topics 1-4 (scientific method, biomolecules, microscopes and cells,


worth 20% of your grade!

DO NOT arrive late

no electronics on your person (phones, ear buds, smartwatches in your bag)

Written Exam: 40-45 questions on written portion

55 questions, 2 points each

One or two stations for practical applications: I will be monitoring you at these stations and giving you points upon satisfactory completion (10-15 points)

when complete: students can either turn their paper in or revisit stations one

at a time for a short period

Revisit the powerpoints for all of the labs

Review the lab manual pages

If we thought it was important enough to put in a powerpoint or cover it in a quiz or do it as an activity, it’s important enough to put on the exam!

Use the study guide found on Canvas

Biology Tutoring (Walker Science room 149)

Tutoring for BSC 110/111:

Monday 8am – 7pm

Tuesday 8am – 11am and 2:30pm – 5pm

Wednesday 8am – 5pm

Thursday 8am – 11am and 2:30pm – 3:30pm

Friday 9am – 10:30am

Tutoring for BSC 107/250/251:

Monday 10am – 12pm

Wednesday 10am – 12pm

Thursday 5:30pm – 7pm

Friday 8am – 12pm

A graduate student will be present who can help with lab reports during the following times:

Tuesday 10am – 12pm and 2pm – 5pm

Wednesday 10am – 12pm and 2pm – 5pm

Thursday 10am – 12pm and 2pm – 5pm

Friday 10am – 12pm

From the syllabus: Students arriving more than 15 minutes late for a lab practical will not be allowed to take the practical during the allotted time. If a suitable excuse is provided to the teaching assistant and approved by the lab coordinator, a make-up lab practical may be scheduled.

In the event a student misses a lab practical due to family emergency, illness, or University function, contact the Teaching Assistant and Lab Coordinator immediately. If the student does not contact the Teaching Assistant and Lab Coordinator within 48 hours of the exam, the student will not be allowed to make up the missed assessment.

In addition, a verified excuse must be obtained from the Assistant to the Vice President of Student Affairs in Union 219 and submitted to the Teaching Assistant and Lab Coordinator within one week of the date of the exam. After review and approval of the excuse, the Teaching Assistant and Lab Coordinator will work with the student to arrange a date and time for a make-up practical. A student is only allowed to make up one lab practical during the semester. If there was no prior contact and/or verified excuse obtained, as explained above, the student will receive a score of zero on any assessment for that day.


Activities: Enzymes Lab

Prepare for the week of the 7th:


Prepare for the week of the 21st:

Fill out Excel sheet with your data

Study for quiz on topics 4 and 5

Prelab for Topic 5: Visualizing Metabolism: Respiration and Photosynthesis

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